Service content and timeline (full service)
Our standard CRISPR/Cas9 GM mouse production service involves three staged service modules.
1. Stage 1: CRISPR design
2~4 weeks dependent on complexity of CRISPR design
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Primer design
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Guide RNA design
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Repair template design (project type 3, 4, 5)
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Order reagent (Cas9 protein, sgRNA, ssODN < 200bp, or gBlock dsDNA< 1.5 kb)
Note:
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Time is from when the agreement and design is finalised with client and reagent order placed
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Not including the donor repair template cloning, synthesis or delivery time
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Optional in vitro or in cell CRISPR validation will take approximately another 2 weeks after the reagent arrive
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QFAGE will email researcher when the CRISPR has been made