Sperm cryopreservation

The maintenance of mouse colonies can be expensive and time consuming. Sperm freezing offers the researcher an alternative to maintenance breeding to preserve a mouse line that is not in current use. Sperm freezing also provides a back-up to preserving the line in the unlikely event that the colony becomes pathogen compromised, has a fertility issue or background/genotype is compromised.

When deciding to cryopreserve a line, the Client will need to choose if embryo or sperm freezing is best suited to the strain.

Mice needs

Costs

Additional costs

If after 2 IVF rounds the cryopreserved sperm is unsuccessful in producing 2 cell embryos, TASQ will contact researcher about supplying additional mice for further cryopreservation. Test thaw fee is applicable for each round of freezing.
Courier costs to UQ animal facility.
Animal agistment of males. Agistment is the weekly charge applied by the animal facilities for the basic care of the animal (contact TASQ for charges).

How to start

Timeline

Additional notes

TASQ will freeze all viable sperm collected.
Additional charges will apply to those lines where males are to be frozen separately
TASQ cannot guarantee any mouse strains’ viability after freezing.
TASQ registration form must be completed prior to service commencement.
Researcher must not cull original colony stock until discussion with TASQ after completion of sperm freezing.
A current AEC certificate and OGTR number must be supplied prior to commencement of service. Any AEC renewals or modifications must be submitted to TASQ.