The maintenance of mouse colonies can be expensive and time consuming. Sperm freezing offers the researcher an alternative to maintenance breeding to preserve a mouse line that is not in current use. Sperm freezing also provides a back-up to preserving the line in the unlikely event that the colony becomes pathogen compromised, has a fertility issue or background/genotype is compromised.

When deciding to cryopreserve a line, the Client will need to choose if embryo or sperm freezing is best suited to the strain.