Full service

For genomic deletions, two pairs of sgRNAs (four in total) flanking the region of interest will be designed and delivered in pairs to delete up to 100 kb of the genome. This strategy could also be used to knock out a gene of interest by deletion key exons or whole genomic region.

Full-service Price: $7056

These projects involve the generation of small, specific changes (e.g. point mutations, deletions, small specific insertions or up to 60bp, or epitope tagging). This involves one or two single sgRNA targeting the locus of interest, and homology-director repair (HDR) via a single-stranded DNA oligonucleotide repair template up to 200 bases.

Full-service Price: $7938

These projects involve generation of large DNA fragment insertions (reporter or selection cassette) or conditional (“floxed”) alleles. These experiments involve either one or two sgRNAs and a large single or double-strand DNA repair template with left and right homology arms and insert sequence (synthesized DNA or plasmid DNA substrate).

Full-service Price: $9040.5

These projects use predesigned guide RNA to knock in your expression cassette as a repair template into Rosa26 safe-harbour sites of the genome.

Full-service Price: $7479

• Project Consultation/CRISPR design and Production

• Microinjection/Electroporation of CRISPR into C57Bl/6 (200eggs)

• Embryo transfers into pseudopregnant females at AIBN SPF Facility

• Tissue Sampling, Genotyping and Sequencing of Progeny (30 samples)

• Agistment until pups are weaned

• Fetus collection from embryo transfer recipient females at requested d.p.c

• Other wild type strains can be used as donors. Additional charges may apply for the purchase of these strains.

• Novel/GM mice can be supplied by the researcher to be used as either the donor female/male or both for zygote production.

• Current AEC and OGTR/IBC must be supplied prior to microinjection work.

• TASQ will transfer all viable injected/electroporated embryos collected and injected. TASQ cannot guarantee that all embryo transfers result in live pups or transgenic founders.

• If first round of injections results in no founder mice, an additional microinjection will be conducted, see below charges for additional session.

• TASQ will not cull any wildtype progeny until a written request is received from the Client.

• For project type 4 and 5, QFAGE will do the donor repair template design. It is assumed the molecular cloning of the donor plasmid will be undertaken by the client’s lab or outsourced to PEF or other companies. Alternatively, a long synthesized single strand DNA (<3kb) could also be ordered (eg. From IDT or Genescript) with price quoted for the client.

(Costs will be dependent on Founder number and colony requirement/ size)

• CRISPR validation in vitro (cutting efficiency) or in mouse MEF cells (editing efficiency) prior to microinjection.

• Colony management for the breeding, production and tissue sampling of next generation breeding and subsequent experimental animals.

• Large scale experimental sex/age matched cohort expansion through IVF

• Courier costs of transgenic progeny to Client if applicable

Our standard CRISPR/Cas9 GM mouse production service involves three staged service modules. Review details of the service content and timeline for this full service.