Fragment Services

Our PCR fragment analysis services can save you time and resources by using high-throughput capillary electrophoresis to analyse your PCR products. The information below shows some of the applications that our CE DNA analysis services can assist with.

PCR assays are commonly used to screen for the presence or absence of a variety of genetic features, e.g. insertions, deletions, and SNPs, in a range of organisms. These assays include techniques such as:

Restriction Fragment Length Polymorphisms (RFLPs)
Amplified Fragment Length Polymorphisms (AFLPs)
Single-stranded conformation polymorphism (SSCPs)
Other custom designed PCRs

The DNA amplified in these assays is often then analysed by running agarose gels which, when you have a high number of samples, can be a very time consuming and labour-intensive process.

Our fragment analysis service can speed up your research by analysing your PCR results in a 96-well plate format using high-throughput CE DNA analysers. Fluorescently labelled PCR products from 50-1100 base pairs can be analysed and differences in fragment size as little as 1 base pair can be detected. Compatible fluorescent dyes include 6-FAM, VIC, NED, and PET. The increased resolution of capillary electrophoresis and the availability of different fluorescent dyes means that you can also multiplex assays, further saving time and reagent costs.

Please see below for more information on submitting samples to us for Fragment Analysis.

Sample Submission

Samples can be submitted in person to Genetic Research Services. A submission form must also be emailed to uqgrs@uq.edu.au before we can process the samples.

Sample Submission Times: 8:00am to 3.30pm (excluding weekends and public/University holidays).

Genetic Research Services Location: Level 7, Chemistry Building (#68), University of Queensland, St Lucia.

Alternatively, samples can be submitted via the internal mail or sent via Australia Post.

Please submit samples for fragment analysis in clearly labelled strip tubes or a labelled 96-well plate. Please submit approx. 10µL for each sample. Samples should be wrapped in alfoil to be protected from the light.

Results

Results

Our Fragment Analysis service runs as needed (excluding public holidays). Samples submitted before 12pm will be reported the following business day.

All fragment FSA files are saved onto a special shared drive set up for our clients. Only people that have approved access for each group will have access to that individual group’s results folder. Results will be made available on the shared drive for a 1 month period, however, the Genetic Research Services maintains an archive of all fragment analysis results and can reissue past results on request.

To analyse your FSA files you can use Peak Scanner (free online software).

Fragment Services

Sample Submission

Results

Enquiries