Cryopreservation comparison table - embryo vs sperm

Can take a few months to acquire all the embryos for storage.

Work is done in one day.

As charges are dependant on the number of females used, costs will vary for each strain. Is more expensive than gamete freezing. For UQ researchers, some strains may be free of charge, this will be dependent on genotype and strain background.

UQ researchers: free of charge

Cheaper than embryo freezing

Het X WT matings= 500

Het x Het matings=300

Homo X WT= 250

Homo X Homo= 250

Collect sperm from 3-5 males, so there are millions.

Thaw assesses viability to live births.

Frozen Sperm IVF Test Thaw is performed. Embryos are cultured to blastocyst to determine viability.

Set cost for rederivation per round. Involves embryo thaw and culture, then an embryo transfer. Cheaper and less labour intensive

Set cost for Mouse IVF per round. Requires donor priming, embryo collection, IVF and culture, then embryo transfer. More expensive and more labour intensive.

When rederived the genotype and phenotype of the resulting progeny will be exactly same as when freezing done.

Only cryopreserves half of genetic make-up of the original strain/background. Future rederivation can only produce a Het (due to IVF with WT eggs).